Figure 4
From: IL-10 modulates DSS-induced colitis through a macrophage–ROS–NO axis
Inflammatory cytokine production by IL-10Rα-deficient macrophages. (a) Colons from IL-10RαMdel and IL-10Rαfl/fl mice, at 7 days after colitis induction, were homogenized and cytokine content measured by ELISA or multiplex assay. Results from individual mice (circles) and cohort means (lines) are plotted. (b) Relative expression levels (mean±1 s.d.) of the indicated mRNAs from macrophages sorted from colon tissue was measured by qRT–PCR. *P<0.05, ***P<0.001. Data are representative of three independent experiments. IL, interleukin; MCP-1, monocyte chemotactic protein-1; qRT–PCR, quantitative real-time reverse-transcriptase–PCR; TGF-β, transforming growth factor-β; TNF-α, tumor necrosis factor-α.
