Figure 5
From: IL-10 modulates DSS-induced colitis through a macrophage–ROS–NO axis
Role of nitric oxide (NO) and arginase in colitis exacerbation. (a) Macrophages were sorted from colons of mice with colitis (day 7) and inducible nitric oxide synthase (iNOS) and arginase expression were measured by quantitative real-time reverse-transcriptase–PCR (qRT–PCR). The ratio of expression in interleukin (IL)-10RαMdel to IL-10Rαfl/fl cells was measured. Mean±1 s.d. is plotted. (b) Colon tissue from mice with colitis (day 7) was homogenized and tissue NOS activity measured using a colorimetric assay. Results from individual mice (circles) and cohort means (lines) are shown. (c) Colitis was induced in IL-10RαMdel and IL-10Rαfl/fl mice with 3% dextran sodium sulfate (DSS). Aminoguanidine hydrochloride (AG) or saline was administered intraperitoneally (i.p.). Mean±1 s.e.m. weight change from day 0 is plotted (n=10/cohort). (d) Rectal bleeding scores measured on day 7 after colitis induction. (e, f) Analyses are similar to c and d except that IL-10RαMdel and IL-10Rαfl/fl mice (n=10/cohort) were treated with S-(2-boronoethyl)-l-cysteine (BEC) or saline by i.p. injection. *P<0.05, **P<0.01, NS, not significant. For experiments in c–f, statistical significance is only shown comparing drug-treated and untreated IL-10RαMdel or IL-10Rαfl/fl mice. Significance levels between IL-10RαMdel and IL-10Rαfl/fl cohorts are not shown. Data are representative of two independent experiments.
