Figure 6
From: IL-10 modulates DSS-induced colitis through a macrophage–ROS–NO axis
Role of reactive oxygen species (ROS) in colitis exacerbation. (a) On day 7 after colitis induction, gated T cells, B cells, dendritic cells (DCs), and macrophages were analyzed by flow cytometry in the lamina propria (LP) or spleen as indicated. Gray line, isotype control staining of interleukin (IL)-10RαMdel cells; dashed black line, IL-10Rαfl/fl cells; solid black line, IL-10RαMdel cells. (b–e) Colitis was induced with 3% dextran sodium sulfate (DSS) in IL-10RαMdel and IL-10Rαfl/fl mice that were treated with N-acetyl-L-cysteine (NAC) with or without aminoguanidine hydrochloride (AG) or saline intraperitoneally (i.p.). Mean±1 s.e.m. weight change is measured. Plots b–e show results for all cohorts, IL-10Rαfl/fl cohorts, IL-10RαMdel cohorts, and a comparison of NAC+AG-treated IL-10RαMdel with untreated or NAC+AG-treated IL-10Rαfl/fl cohorts, respectively. *P<0.05, **P<0.01 comparison of AG+NAC vs. untreated mice in panels c and d, and AG+NAC-treated IL-10Rαfl/fl vs. IL-10RαMdel cohorts in panel e. °P<0.05, °°P<0.01 for NAC vs. untreated cohorts in panels c and d. ▵▵P<0.01 for AG+NAC vs. NAC in panel d. (f) Rectal bleeding scores on day 7 after DSS treatment. *P<0.05, ***P<0.001, NS, not significant. Comparisons are only shown for treated vs. untreated IL-10Rαfl/fl or IL-10RαMdel cohorts and not between IL-10Rαfl/fl and IL-10RαMdel mice. Data are representative of two independent experiments.
