Figure 2

Mucus staining in Carnoy’s fixed ileal sections of wt (a, b) and REG3γ−/− (c, d) mice. PAS/Alcian Blue staining of ileum (a, c). Immunofluorescent detection of MUC2 (b, d) using an anti-MUC2 antibody and goat-anti-rabbit Alexa 488. Arrow in A indicates the continuous layer of mucus overlaying the villi tips. Representative images are shown in each panel. wt, wild-type.