Figure 4
From: The TNF-family cytokine TL1A promotes allergic immunopathology through group 2 innate lymphoid cells
Chronic, but not acute exposure to TL1A can induce interleukin-13 (IL-13) expression in innate lymphoid cell (ILC). (a) ICOS and IL-13eGFP expressing cells were quantitated in mesenteric lymph node (mLN) following four consecutive doses over 4 days of phosphate-buffered saline (PBS), IL-25 (2 μg per dose), IL-33 (0.5 μg per dose), or TL1A (5 μg per dose) given to Il13+/eGFP reporter mice. Dot plots are gated on lineage (CD3CD4CD8CD19CD11bCD11cFcεR1)-negative cells. Examples are representative of 3–5 mice per group. (b) ICOS and IL-13eGFP expressing cells were quantitated in mLN and lamina propria from Il13+/eGFP reporter mice untreated, given 2 μg of IL-25 for four consecutive days, or crossed to CD2-TL1A transgenic (Tg) mice. (c) il25 and il33 mRNAs were measured by qRT-PCR in the ileum of CD2-TL1A Tg mice and normalized to the average levels found in wild-type (WT) age-matched controls and analyzed with Mann–Whitney test (**P<0.01). See also Supplementary Figure S3.
