Figure 6

DR3 is required for group 2 innate lymphoid cells (ILC2) expansion and lung pathology during allergic lung disease. (a) Percentage of Lin⁻ (CD3,CD4,CD8,CD11b,CD11c,B220,GR1,Ter119,NK1.1) Thy1⁺ ILC gated in CD45⁺ population in the lungs of mice of the indicated genotype immunized and challenged with ovalbumin or phosphate-buffered saline (PBS) control. (b) Quantitation of Lin⁻ Thy1⁺ cells from the lungs of mice immunized and challenged as in (a). Each point represents one mouse with mean±s.e.m., with significance from the unpaired t-test (***P<0.001). (c) Percentage of Lin⁻ (CD3,CD4,CD8,CD11b,CD11c,B220,GR1,Ter119,NK1.1) Thy1⁺ ILC gated in CD45⁺ population in the lungs of mice of the indicated genotype intranasally challenged with papain or control (PBS). (d) Quantitation of Lin⁻ Thy1⁺ cells from the lungs of mice immunized and challenged as in (c). (e) Numbers of eosinophils in the bronchoalveolar lavage (BAL) of these mice, calculated from yield and flow-cytometric assessment of BAL as described in Methods. (f) Representative histological sections from the lungs of mice challenged with Papain as in (c). Hematoxylin and eosin (H&E) staining, scale bar=50 μm. Quantitation of immunopathology in lung histological sections examined from groups of mice treated as in (c) is shown in (g). Scoring was performed as described in Methods. Each symbol represents the score of one mouse. Expression of il13 (h) and muc5ac (i) mRNA in lung tissue isolated from the mice treated as in (c) measured by qRT-PCR and normalized to the average levels found in wild-type (WT) age-matched controls. Data were analyzed with Mann–Whitney test (*P<0.05, **P<0.01, ***P<0.001).

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