Figure 6
From: Mouse and human Notch-1 regulate mucosal immune responses
Effect of Notch-1 knockdown (KD) on epithelial proinflammatory responses. (a) Interleukin-8 (IL-8) mRNA expression was assessed in control and Notch-1 short hairpin (shRNA)–treated Caco-2 cells. Cells were incubated with or without tumor necrosis factor-α (TNFα) for 4 h. (b) IL-8 secretion of cell supernatants from control or Notch-1 shRNA-treated Caco-2 cells was quantified after a 2-h TNFα pulse followed by a 2-h rest by Cytometric Bead Array (CBA). Data represent the mean±s.e.m. of at least four independent experiments. P<0.05 was considered statistically significant. (c) Immunoblotting for p-p38, IκB-α, and β-actin in lysates obtained from TNF-α-treated control or Notch-1 shRNA Caco-2 cells (representative of three experiments).
