Figure 7
From: Mouse and human Notch-1 regulate mucosal immune responses
Effect of tumor necrosis factor-α (TNFα)–stimulated Notch-1 knockdown (KD) Caco-2 cell on peripheral blood T-cell subsets. (a) Flow cytometry analysis of FoxP3+ CD4 T cells and interferon-γ (IFNγ)+ CD4 T cells from anti-CD3/CD28-stimulated peripheral blood mononuclear cells (PBMCs) from healthy individuals incubated with TNFα-stimulated control short hairpin (shRNA) or Notch-1 KD Caco-2 cell supernatants. (b) Interleukin (IL)-6, -10, and IFNγ cytokine secretion assessed by Cytometric Bead Array (CBA) from culture supernatants of anti-CD3/CD28-stimulated PBMCs from healthy individuals incubated with TNFα-stimulated control shRNA and Notch-1 KD Caco-2 cell supernatants; n=5–8 individuals. P<0.05 was considered statistically significant.
