Figure 1
From: Intestinal bacteria induce TSLP to promote mutualistic T-cell responses
Thymic stromal lymphopoietin (TSLP) expression is induced by commensal bacteria in the colon. (a) Real-time reverse transcriptase–PCR (RT-PCR) analysis of TSLP mRNA expression in freshly isolated tissues from germ-free or specific pathogen-free (SPF) mice. (b) Real-time RT-PCR analysis of TSLP mRNA expression in the epithelial cell (EC) or colonic lamina propria (cLP) fractions from altered Schaedler flora (ASF) and SPF. (c) Real-time RT-PCR analysis of TSLP mRNA expression in bone marrow–derived dendritic cells from C57BL/6 or TSLPR−/− mice. Results are normalized to β-actin or GAPDH (glyceraldehyde 3-phosphate dehydrogenase) and are shown from one representative experiment of two independent experiments (n=4–5 per group). LPS, lipopolysaccharide. *P<0.05.
