Figure 7
From: Intestinal bacteria induce TSLP to promote mutualistic T-cell responses
CD4+ T cells and regulatory T cells express the high-affinity receptor for thymic stromal lymphopoietin (TSLP) and respond directly to this cytokine. Representative histograms of interleukin-7R (IL-7R) and TSLP receptor (TSLPR) expression in (a) CD4+ and (b) CD4+CD25+FoxP3+ cells from colonic lamina propria (cLP) and mesenteric lymph nodes (MLN) of day 28 ASF-colonized C57BL/6 or TSLPR−/− mice. Fluorescence Minus One Control (here indicated as control) was used for TSLPR and IL-7Rα staining. Results are shown from one experiment and are representative of two independent experiments (n=3–4 per group). Representative plots of TSLPR−/− (CD45.2+) and wild-type CD45.1 (CD45.2−) FoxP3+ cells isolated from (c) cLP and (d) MLN of day 28 ASF RAG−/− mice reconstituted with CD4+ splenocytes from SPF TSLPR−/− or wild-type CD45.1 mice. Quantification (e) of TSLPR−/− (CD45.2+) and wild-type CD45.1 (CD45.2−) FoxP3+ cells isolated from (c) cLP and (d) MLN. Results are shown from one experiment and are representative of two independent experiments (n=5, per group per experiment; mice were pooled for cLP). **P<0.01.
