Figure 1
Definition of cell populations in bronchoalveolar lavage (BAL) by flow cytometry. The major cell populations in whole BAL were defined initially by their forward scatter (FSC) and side scatter (SSC) characteristics. The identities of the cell populations were then confirmed by demonstrating the gated cell populations labeled with anti-CD206 (macrophage marker) and anti-CD3 (T-lymphocyte marker), respectively. (a) Representative pseudo-color plot showing the gating strategy for macrophage and lymphocyte populations. (b) Representative dot plot showing the major cell populations in whole BAL (macrophages=blue and lymphocytes=red). (c) Labeling of gated cell populations with macrophage and T-lymphocyte markers, and (d) the proportion of small and large alveolar macrophages (AMs) in BAL from HIV-1-infected and HIV-1-uninfected individuals. Data were analyzed using the Mann–Whitney U-test; black horizontal bars represent medians (HIV−, n=19; HIV+, n=22).
