Figure 4
Small alveolar macrophages (AMs) are the dominant macrophage population harboring HIV in the alveolar space. Adherent AMs isolated from bronchoalveolar lavage (BAL) cells of healthy, HIV-infected individuals were stained with fluorescence in situ hybridization (FISH) probes against HIV-gag mRNA and analyzed by flow cytometry. AMs were identified by their forward scatter (FSC) and side scatter (SSC) characteristic properties and HIV-infected cells were identified by their HIV-gag mRNA positivity. (a) Representative flow cytometry pseudo-color plots of adherent AMs from HIV-1-uninfected and HIV-1-infected participants. (b) Proportions of HIV-infected AMs in adherent BAL cells from individuals with chronic HIV infection Data were analyzed using the Mann–Whitney U-test; black horizontal bars represent medians (HIV−, n=6; HIV+, n=14).
