Figure 6
Surface marker expression by HIV-infected alveolar macrophages (AMs) in whole bronchoalveolar lavage (BAL) from HIV-1-infected participants. Whole BAL cells stained with anti-CD206 fluorescein isothiocyanate (FITC), anti-HLA-DR AF700, anti-CD45 PE, and anti-CD71 PE-Cy5 for surface marker expression, and HIV-gag Quasar 670 fluorescence in situ hybridization (FISH) probes to detect HIV-infected cells were analyzed by flow cytometry. AMs were identified by their forward scatter (FSC) and side scatter (SSC) characteristics and the expression of CD206, HLA-DR, CD45 and CD71 by HIV-infected and HIV-uninfected AMs from the same individual was determined. (a) Representative histograms showing expression of CD206, HLA-DR, CD45 and CD71 by HIV-infected and HIV-uninfected AMs. (b) Expression of CD206, HLA-DR, CD45 and CD71 by HIV-infected and HIV-uninfected AMs. Data were analyzed by the Wilcoxon matched-pairs signed-ranked test (n=6).
