Figure 2
From: Chronic dry eye disease is principally mediated by effector memory Th17 cells
T cells from chronic dry eye disease (DED) mice induce the most severe disease in naive recipients. (a) Schematic study design of adoptive transfer experiment. Total T cells were isolated from normal, acute, and chronic DED (n=3–5 mice/group). Each naive recipient was then injected intravenously with 1 × 106 cells. Recipients were challenged in the controlled-environment chamber for 12 days without use of scopolamine. (b) Disease severity comparison among the recipients transferred with the different T cells. The mean corneal fluorescein staining score±s.e.m. for each group (n=16 eyes) is shown. *P<0.05; **P<0.01 compared with normal-T-cell recipients. †P<0.05; ‡P<0.01 compared with acute DED-T-cell recipients. (c) T-cell response in the recipient draining lymph nodes (LNs). Draining LNs were harvested at day 6 and analyzed for IFN-γ and IL-17 levels by enzyme-linked immunosorbent assay (n=3 mice per group). Data shown are mean±s.e.m. *P<0.05. Groups are labeled as follows: Normal T-cells, recipients of T cells from normal mice; Acute DED-T cells, recipients of T cells from acute DED mice; and Chronic DED-T cells, recipients of T cells from chronic DED mice.
