Figure 3

T cells from chronic dry eye disease (DED) show increased effector memory T helper type 17 population. Draining lymph node cells and spleen cells from normal, acute, and chronic DED mice were analyzed by flow cytometry. Representative results from spleens are shown, with similar findings in draining lymph nodes. (a) CD62L vs. CD44 expression on gated CD4⁺ cells is presented. Four T-cell populations were differentiated:¹⁶ CD62L⁺CD44^lo naive T cells (T_N), CD62L⁻CD44^lo effector T cells (T_E), CD62L⁺CD44^hi central memory T cells, and CD62L⁻CD44^hi effector memory T cells (T_M). Percentages indicate frequencies of each cell population. (b) Sorted T_N from normal, T_E from acute, and T_M from chronic DED were further analyzed for interferon (IFN)-γ and interleukin (IL)-17 expression. Frequencies of IFN-γ⁺ or IL-17⁺ cells in each individual populations are summarized as mean±s.e.m. of three independent experiments shown in the bar graph. **P<0.01 compared with T_N; ^‡P<0.01 compared with T_E.

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