Figure 3
From: Lung epithelial cells are essential effectors of inducible resistance to pneumonia
Myeloid differentiation primary response gene 88 (MyD88) and interleukin-1 receptor-associated kinase 4 (IRAK4) are required for Pam2-ODN-induced resistance. (a) Myd88−/− and wild-type littermates were inhalationally challenged with Pseudomonas aeruginosa grown to mid-log phase with or without aerosolized Pam2-ODN (oligonucleotide) treatment 24 h before infection. Left, survival (N=10 mice/group); right, lung bacterial burden immediately after infection (N=3 mice/group). (b) Irak4−/− and wild-type mice inhalationally challenged with P. aeruginosa with or without Pam2-ODN pretreatment 24 h before infection. Left, survival (N=10 mice/group); right, lung bacterial burden immediately after infection (N=3 mice/group). (c) Irak4−/− and wild-type mice inhalationally challenged with Streptococcus pneumoniae with or without Pam2-ODN pretreatment 24 h before infection. (d) Irak4−/− and wild-type mice inhalationally challenged with influenza A virus (H3N2) with or without Pam2-ODN pretreatment 24 h before infection. (*P<0.0001 wild-type+Pam2-ODN vs. knock-out+Pam2-ODN; **P<0.0001 wild-type+PBS (phosphate-buffered saline) vs. wild-type+Pam2-ODN; †P<0.02 wild-type+PBS vs. wild-type+Pam2-ODN). CFU, colony-forming units.
