Figure 2
Influence of AKB-4924 treatment on pyrogen levels in trinitrobenzene sulfonic acid (TNBS) colitis. AKB-4924 (5.0 mg kg−1 in cyclodextrin vehicle) or vehicle-treated animals were killed on day 2, 3, or 7 relative to TNBS or EtOH rectal lavage. Blood was collected by cardiac puncture and serum assayed in triplicate for each animal by multiplex enzyme-linked immunosorbent assay (ELISA) for (a) interleukin (IL)-1β, (b) IL-6, (c) tumor necrosis factor (TNF)-α, and (d) IL-10. On day 7, whole-colon tissue was homogenized in lysis buffer and assayed by ELISA for (e) IL-1β, (f) IL-6, (g) TNF-α, and (h) IL-10. N=6, *P<0.05, **P<0.01 two-tailed Student’s t-test.
