Figure 4
Influence of AKB-4924 on systemic bacteremia in trinitrobenzene sulfonic acid (TNBS) colitis. (a) Recovery of viable bacteria translocated to blood and extra-intestinal organs (liver, kidney, spleen, and mesenteric lymph nodes (MLN) in TNBS colitis animals after treatment with vehicle or AKB-4924 (5 mg kg−1). Bacterial counts are expressed in colony-forming units per gram tissue (CFU g−1), except for ^blood where counts are expressed as CFU ml−1. (b) Murine neutrophils and macrophages were isolated by peritoneal lavage and incubated with 10 μM AKB-4924 (AKB) or incubated in hypoxia (Hx; pO2, 20 torr for 6 h) or normoxia (Nx) and assessed for the ability to phagocytose fluorescein isothiocyanate-labelled Escherichia Coli. N=5, *P<0.05, **P<0.01 two-tailed, Student’s t-test.
