Figure 6
Influence of AKB-4924 on animals with active trinitrobenzene sulfonic acid (TNBS) colitis. AKB-4924 (0 or 5.0 mg kg−1) was administered subcutaneously on day of peak weight loss (day 2) relative to TNBS or EtOH intrarectal lavage. Cyclodextrin was administered as a control. (a) Weight was measured daily as an indicator of disease severity and normalized relative to initial weight and control animals. On day 7, animals were killed and (b) colons were excised and assessed for shortening, and (c) total cell populations in mesenteric lymph nodes (MLN) were counted using a haemocytometer. (d) Disease activity was assessed as sum of scoring (0–3) for colon thickening (colon weight/length), occult blood, fecal pellet consistency, and percentage of weight loss. (e) Temperature was measured by IR thermometer at 24-h intervals from first AKB-4924 administration until killing. N=6, *P<0.05, **P<0.01, analysis of variance (a, e), two-tailed, Student’s t-test (b, c, d).
