Figure 3
From: Microfold (M) cells: important immunosurveillance posts in the intestinal epithelium
Retrospective comparison of chemokine gene expression by M cells and the follicle-associated epithelia (FAE). (a) Heat map showing the mean expression of profile of multiple chemokine-encoding probe sets in the samples of Peyer’s patch (PP) M cells, cholera-toxin-induced (CT-stim.) villous M cells and ileum intestinal epithelial cells (GSE7838),25 FAE, M cells,26 and ileum (GSE13908).68 These data were performed on Affymetrix MOE430_2 mouse genome expression arrays (Affymetrix, Santa Clara, CA). Each column represents the mean probe set intensity (log2) for all the samples from each source (n=2–4). Significant differences between groups were sought by analysis of variance. P-values for those genes which were expressed significantly (P<0.05) within the FAE and by M cells at levels >two fold when compared with the villous epithelium are indicated. (b) Effect of receptor activator of nuclear factor-κB ligand (RANKL)-stimulation on the expression of chemokine-encoding genes in the villous epithelium. These data (GSE37861)2 were performed on Affymetrix mouse gene 1.0 ST expression arrays (equivalent chemokine-related probe sets are shown). Each column represents the mean probe set intensity (log2) for all the samples from each source (n=3). P-values for those genes that were significantly upregulated >two fold at day 3 after RANKL-treatments when compared with controls (day 0) are indicated. Representative probe set are shown when multiple probe sets for a gene were present on the arrays. NS, not significant.
