Figure 1
From: Basophils control T-cell responses and limit disease activity in experimental murine colitis
Characterization of basophils after reconstitution of SCID mice with CD4+CD62L+ T cells. (a–c) SCID mice (n=6/group) were reconstituted with 2 × 106 CD4+CD62L+ T cells from BALB/c mice (white bars) or received just phosphate-buffered saline without T cells (grey bars) and analyzed 7 days later. (a) Plasma cytokine levels were quantified by cytometric bead array. (b) The plasma level of interleukin (IL)-3 was measured by in vivo cytokine capture assay. (c) By staining with antibodies against FcɛR1 and CD49b, the numbers of FcɛR1+ and FcɛR1++ basophils were quantified in the spleen of SCID mice 7, 14, 25, and 35 days after reconstitution with CD4+CD62L+ T cells from BALB/c mice. (d) SCID mice were adoptively transferred with 0.5 × 106 CD4+CD62L+ T cells from BALB/c mice (white bars) or IL-3-deficient mice (black bars) (nine mice per group). The control group received no T cells (grey bars; 10 mice per group). After 8, 16, 29, and 84 days, basophils were quantified in the peripheral blood by flow cytometry with counting beads. Data are represented as mean±s.e.m. IFN, interferon; TNF, tumor necrosis factor; WT, wild type.
