Figure 2

Migration of T cells to the lung is required for protection against primary Nippostrongylus brasiliensis (NB) infection. (a) Mice were treated daily with FTY720 beginning 1 day before infection and killed either at day 2 or day 10 post infection (PI). (b) Impaired resolution of infection in FTY720-treated mice was established by quantification of intestinal adult worm burdens at day 10 post infection. Flow cytometric analysis of cell suspension of whole lung stained for (cA) CD3⁺CD4⁺, CD3⁺CD8⁺, (cB) CD4⁺CD44^loCD62L^hi naive, CD4⁺CD44^hiCD62L^lo effector memory, and (d) CD4⁺CD62L^loT1/ST2⁺ T cells. Lung cells were re-stimulated with N. brasiliensis excretory secretory proteins for 5 days, and (e) secretion of interleukin (IL)-13 was detected by enzyme-linked immunosorbent assay. Data are representative of two individual experiments. N=6–10 mice per group. *P<0.05, **P<0.01, ***P<0.001.

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