Figure 4
Lung-resident T cells are independent of tertiary lymphoid structures. Secondary Nippostrongylus brasiliensis (NB)-infected mice were treated weekly with 100 μg MOPC21 isotype or LTβR-Ig (lymphotoxin beta-receptor fusion protein) beginning 3 days before primary infection. (a) Primary infections were cleared at day 7 by Ivermectin treatment, and mice were subsequently reinfected at day 28. (b) Efficiency of the treatment was confirmed by flow cytometric analysis of B220+CD23loCD21hi marginal zone B cells. (c) This was further confirmed by staining spleens with either CD3 (blue)/IgD (brown) or peanut agglutinin (PNA) (blue)/IgD (brown) to look at marginal zone B cells and germinal centers, respectively. Arrows indicate location of these structures. (d) Adult worm burdens were quantified at day 5 post secondary infection to evaluate ability to control infection. Numbers in the flow cytograms indicate the percentage of marginal zone B cells cells relative to lymphocytes. Data are representative of one individual experiment. N=6 mice per group. **P<0.01. IP, intraperitoneal; PI, post infection.
