Figure 7
Lung-resident T cells require interleukin (IL)-4Rα expression for optimal immunity to Nippostrongylus brasiliensis (NB) reinfection. IL-4Rα-/loxp and LckcreIL-4Rα-/loxp were treated with FTY720 daily or left untreated. (a) Primary infections were cleared at day 7 by Ivermectin treatment, and mice were given a secondary N. brasiliensis infection at day 28 and killed at 5 days post infection (PI). (b) Intestinal worm burdens were assessed to determine protection kinetics. (c) CD3+CD4+ CD3+CD8+, CD3+CD4+CD44loCD62Lhi naive and CD3+CD4+CD62LloT1/ST2+ T-cell numbers were quantified by flow cytometric analysis of single-cell suspensions of whole lung. (d) Whole-cell preparations of lung were re-stimulated with N. brasiliensis excretory secretory proteins for 5 days, and cytokine secretion for IL-13 was detected by enzyme-linked immunosorbent assay . Data are representative of two individual experiments. N=6 mice per group. *P<0.05, **P<0.01.
