Figure 2
Mucosal-to-serosal epithelial transport of transferrin (Tf) and human secretory immunoglobulin A (hSIgA) in tyrphostin A8-treated mice. (a) Comparison by confocal microscopy of CD71 expression (green staining) in duodenal sections from Balb/c mice either untreated (left panel) or 48 h after intraperitoneal treatment with tyrphostin A8 (TyrA8). Cell nuclei are stained in blue with DAPI (4,6-diamidino-2-phenylindole). One representative set of images out of five comparable observations is shown. (b) Analysis by multiphotonic video microscopy of apical binding and transcellular transport of Tf (green) and human SIgA (red) in duodenal segments from Balb/c mice either untreated (left panel) or 48 h after TyrA8 treatment (right panels). In TyrA8-treated mice, images are shown at 5-, 10-, and 20-min time points after the intraluminal injection of Tf and SIgA. Image of a duodenal segment from a control mouse at the 20-min time point is shown. Red arrows highlight co-localizing hSIgA and Tf entering enterocytes. One representative set of images out of four comparable experiments is shown (see also Supplementary movies online).
