Figure 1
From: TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica
CCL20 is secreted in a Toll-like receptor 1 (TLR1)-dependent manner from intestinal cells. Levels of CCL20 protein (a) and messenger RNA (mRNA; b) from various mucosal tissues 3 days after oral Y. enterocolitica infection. Data are pooled from two independent experiments (n=4–6 mice per group). (c) Levels of CCL20 mRNA in intestinal epithelial cells (IECs; CD13+CD45-) and lamina propria immune cells (LPCs; CD13-CD45+) sorted from wild-type (WT) and TLR1−/− mice. Purified cells were stimulated with TLR2/1 ligand, TLR2/6 ligand, and Y. enterocolitica (Ye). Data are the average from three to four individual mice. (d) Level of CCL20 from Caco-2 cells transfected with control vector (control), WT TLR1 (I602I), or TLR1 containing a single-nucleotide polymorphism (I602S) 18 h after stimulation with TLR ligands or Y. enterocolitica lysate (Ye). Data are pooled from three independent experiments. (n=6). *P<0.05, **P<0.01, ***P<0.001. Student’s unpaired t-test. MLN, mesenteric lymph node.
