Figure 2
From: TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica
CCL20 production is dependent upon Toll-like receptor 1 (TLR1) signaling and not invasion or type III secretion system (T3SS). (a) Number of migrated cells toward supernatants collected from Caco-2 epithelial cells transfected with wild-type (WT) TLR1 (I602I) and stimulated with wild-type Y. enterocolitica (8081), Y. enterocolitica lacking LcrV (ΔlcrV), T3SS (Δysc), or invasin (ΔinvA). Recombinant human CCL20 (rCCL20) and neutralization of CCL20 (aCCL20) were used as controls. Data are the mean±s.e.m. of pooled from three independent experiments. *P<0.01, **P<0.001 (Student’s unpaired t-test). (b) In vivo production of CCL20 from IECs of TLR1−/− or wild-type littermate control mice infected with 1 × 106 Y. enterocolitica or mutants after 72 h. Data are the mean±s.e.m. of six individual mice pooled from two independent experiments. *P<0.01, **P<0.001 (Student’s unpaired t-test).
