Figure 4
From: TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica
Toll-like receptor 1 (TLR1) and CCL20 are important for recruitment of CCR6+ CD11c+ cells to the Peyer’s patch (PP) during oral Y. enterocolitica infection. Flow cytometric analysis of dendritic cell (DC) population in PP of anti-CCL20-treated (a) and TLR1−/− (b) mice. Data are representative of five individual mice pooled from two experiments. (c) Total cell number of CCR6+CD11c+ cells. (d) Bone marrow chimera’s were performed by reconstituting TLR1−/− mice with wild-type (WT) hematopoietic cells (WT→TLR1−/−) and by reconstituting WT mice with TLR1−/− hematopoietic cells (TLR1−/−→WT). CCR6+ DC in the PP of the bone marrow chimera’s were identified by flow cytometric analysis (left) and total cell count (right) 3 days after infection with Y. enterocolitica. Data are pooled from two independent experiments (n=3–6 mice per group). *P<0.05, **P<0.01, ***P<0.001 (Student’s unpaired t-test).
