Figure 1
D-series resolvins RvD1 and aspirin-triggered RvD1 (AT-RvD1) reduce histamine (His)-stimulated increase in [Ca2+]i in conjunctival goblet cells. Block of histamine-stimulated increase in [Ca2+]i in human (a–d) and rat (e–h) goblet cells. Increase in [Ca2+]i stimulated by histamine over time is shown in a pseudo color image of [Ca2+]i from fura-2 loaded single goblet cells in a and e. Intracellular Ca2+ response over time, in response to increasing concentrations of histamine (10−9–10−5 M) from three experiments (human) or a representative experiment (rat) is shown in b and f top panel. Peak [Ca2+]i over basal for each concentration of histamine from three experiments is shown in b and f bottom panels. *P<0.05 (vehicle vs. histamine). Action of RvD1 and AT-RvD1 exposure on histamine (10−5 M)-stimulated increase in [Ca2+]i in human (c–d) and rat (g–h) goblet cells. Pseudo color image of single goblet cells is shown in c and g; Ca2+i response over time is shown in d and h top panels in response to histamine (10−5 M) alone or histamine after 30 min exposure with RvD1 (10−8 M) or AT-RvD1 (10−8 M) from a representative experiment. Peak Ca2+ response (d and h bottom panels) to increasing concentrations of RvD1 (open circles) and AT-RvD1 (closed circles) is shown from three experiments. *P<0.05 (histamine vs. resolvin plus histamine).
