Figure 10
D-series resolvin RvD1 uses protein kinase C (PKC) and β-adrenergic receptor kinase 1 (βARK1) to block histamine (His) activation of the H1 receptor in Chinese Hamster Ovary (CHO) cells and conjunctival goblet cells. (a, b) Action of PKC inhibitor calphostin C (calC) (10−7M) (a) or βARK1 inhibitor (10−7 M) (b) on RvD1 (10−8 M) block of histamine (10−5 M) on the [Ca2+]i in fura-2 loaded CHO cells that had been transiently transfected with the H1 and GPR32 (G protein-coupled receptor 32) receptors. (c, d) Action of PKC inhibitor Ro-317549 (10−7 M) (c) or βARK1 inhibitor (10−7 M) (d) on RvD1 (10−8 M) block of histamine dimaleate (His Dim; 10−6 M) on the [Ca2+]i in fura-2 loaded rat goblet cells. Intracellular [Ca2+]i over time (upper panels in a and b) or peak [Ca2+]i (lower panels in a and b) in response to histamine (10−5 M), RvD1 (10−8 M) added 30 min before addition of histamine; calphostin C, or βARK inhibitor (10−7 M) added 45 min before histamine; or calphostin C or βARK inhibitor (10−7 M) added 15 min before addition of RvD1 (10−8 M), which was added 30 min before histamine. Intracellular [Ca2+]i over time (upper panels in c and d) or peak [Ca2+]i (lower panels in c and d) in response to histamine dimaleate (10−6 M), RvD1 (10−8 M) added 30 min before addition of histamine dimaleate; Ro-317549 or βARK inhibitor (10−7 M) added 45 min before histamine; or Ro-317549 or βARK inhibitor (10−7 M) added 15 min before addition of RvD1 (10−8 M), which was added 30 min before histamine dimaleate. In all, 17–20cells were selected for each experimental condition.
