Figure 3
D-series resolvins RvD1 and aspirin-triggered RvD1 (AT-RvD1) reduce histamine (His)-stimulated increase in extracellular signal–regulated kinase 1/2 (ERK1/2) activity in conjunctival goblet cells. Block of histamine-stimulated activation of ERK1/2 in rat conjunctival goblet cells. (a) Concentration- and (b) time-dependence of histamine-stimulated increase in ERK1/2 activity measured by western blotting using phosphospecific and total activity antibodies in a representative experiment (left panel) and a mean of three experiments (right panel) *P<0.05 (vehicle vs. histamine). Panel a indicates histamine used for 5 min and panel b indicates histamine used at 10−5 M. (c) Represents action of 30-min exposure of RvD1 (open circles) or AT-RvD1 (closed circles) on 5-min histamine (10−5 M)-stimulated increase in ERK1/2 activity in a representative experiment using RvD1 (top left panel) and AT-RvD1 (bottom left panel) and a mean of three experiments (right panel). *P<0.05 (histamine vs. resolvin plus histamine).
