Figure 9
D-series resolvin RvD1 uses the GPR32 (G protein-coupled receptor 32) receptor to block histamine (His) activation of the H1 receptor by phosphorylating the H1 receptor in Chinese Hamster Ovary (CHO) cells. (a–d) Response of [Ca2+]i to histamine (10−5 M), RvD1 (10−8 M) or 30-min RvD1 exposure followed by histamine in fura-2 loaded CHO cells that had been transiently transfected with (a) H1 and GPR32 receptors, (b) H1 receptor alone, (c) the GPR32 receptor alone, or (d) mock transfected. Panels a and b indicate intracellular [Ca2+]i over time (upper panels) or peak [Ca2+]i (lower panels) in response to histamine (10−5 M), RvD1 (10−8 M), or addition of RvD1 30 min before addition of histamine. Panels c and d show peak [Ca2+]i in response to histamine, RvD1, or addition of RvD1 30 min before addition of histamine. (e) Intracellular [Ca2+]i over time (upper panel) or peak [Ca2+]i (lower panel) in response to histamine (10−5 M), RvE1 (10−8 M), or addition of RvE1 30 min before addition of histamine. AT-RvD1, aspirin-triggered RvD1.
