Figure 1
From: Contrasting immune responses mediate Campylobacter jejuni-induced colitis and autoimmunity
End point cytokine, antibody, and colonic cellular infiltration analysis. IL-10−/− mice were inoculated with C. jejuni 11168 or TSB and killed at humane end point or 35 days after inoculation. (a) Colon homogenate ELISA. IL-12p70, tumor necrosis factor (TNF)-α and IL-17F were only detected in colons of 20% of the mice and are not shown. (b) Colon CD3 and F4/80 immunohistochemistry, at the time of necropsy, the ileocecocolic junction (junction of ileum, cecum, and colon) was fixed in formalin and embedded in paraffin. Its sections were stained for CD3 or F4/80 and the number of positively staining cells in the colonic lamina propria were quantified in 10 random HPFs. (c) Plasma cytokine ELISA. Other cytokines from Figure 1a were not detectable in majority of mice from infected/control group, and are not shown. (d) Plasma C. jejuni-specific antibody analysis. Anti-C. jejuni IgM and IgA was detectable but did not change significantly and is not shown. Data represented are one of three independent experiments with 8–10 mice per group. Bar indicates the median, Mann–Whitney U-test. ELISA, enzyme-linked immunosorbent assay; HPF, high-powered field; IFN, interferon; IL, interleukin; NS, not significant; OD, optical density; TSB, Trypticase Soy Broth.
