Figure 10
From: Contrasting immune responses mediate Campylobacter jejuni-induced colitis and autoimmunity
Whole splenocyte and epithelial cell challenge by gentamicin killing assay. Whole splenocytes (a) or adherent splenocytes (b) from naive C57BL/6 wild-type (wt) mouse were challenged with indicated strains and MOIs in antibiotic-free R10 media. Gentamicin was added after one hour, and cultures were further incubated for 72 h for whole or 24 h for adherent splenocytes, upon which supernatants were clarified and analyzed for indicated cytokines by ELISA. IL-17, IL-4, and IL-23 were not detectable with whole splenocytes while IL-12p70 was only detectable with adherent cell culture. (c) Invasion and IL-6 elicitation by the indicated strains was assessed on young adult mouse colon (YAMC) cells at MOI of 100. Data represent mean±s.e.m. of three wells and analyzed by two-way analysis of variance (ANOVA) for splenocytes or one-way ANOVA for epithelial cells followed by Bonferonni’s post test. Only comparisons with respect to 11168 for splenocytes or HB93-13 for epithelial cells are shown. One of three independent replicates is shown. ELISA, enzyme-linked immunosorbent assay; IFN, interferon; IL, interleukin; MOI, multiplicity of infection; TNF, tumor necrosis factor. ****P⩽0.001.
