Figure 2
From: Contrasting immune responses mediate Campylobacter jejuni-induced colitis and autoimmunity
Temporal analysis of colon leukocyte and C. jejuni-specific plasma antibody. Interleukin (IL)-10−/− mice were inoculated with C. jejuni or Trypticase Soy Broth (TSB), and killed at indicated days after inoculation. At the time of necropsy, the ileocecocolic junction (junction of ileum, cecum, and colon) was fixed in formalin and embedded in paraffin. (a) Hematoxylin and eosin (H&E) sections were scored in blinded manner. (b) Colon leukocytes were prepared and analyzed for indicated populations by flow cytometry. Dead/dying cells were excluded on the basis of forward and side scatter. All cells were gated on CD19-gate. (c) Mice were bled at time of necropsy, and serum was analyzed for indicated antibody subtypes reactive against C. jejuni antigen. Anti-C. jejuni IgM and IgA was detectable but did not change significantly for any time point and is not shown. (d) C. jejuni colonization was measured in the feces at the time of necropsy by quantitative-PCR. (e) Representative photomicrographs from H&E-stained proximal colon sections. Data are represented as mean±s.e.m.; two independent experiments; 5–8 mice per group per time point. Infected group for each time point was compared with control group pooled for each time points by Kruskal–Wallis test and Dunn’s post test. OD, optical density.
