Figure 3
From: Contrasting immune responses mediate Campylobacter jejuni-induced colitis and autoimmunity
Temporal cytokine analysis of colon and MLN. IL-10−/− mice were inoculated with C. jejuni (open circles) or Trypticase Soy Broth (TSB; filled circles) and killed at indicated day after inoculation. (a) In all, 5 mm of proximal colon or (b) 2.5 × 106 red blood cell-depleted cells from mesenteric lymph node (MLN) were incubated in 0.5 ml tissue culture media for 48 h. Supernatant media was clarified and analyzed for indicated cytokines, as well as interleukin (IL)-1β, IL-4, IL-23, and IL-13, which were not detectable. IL-12p70 was detectable from MLN but not the colon. Data are represented as mean±s.e.m.; two independent experiments; 5–8 mice per group per time point. Infected group for each time point was compared with control group pooled for each time points by Kruskal–Wallis test and Dunn’s post test.
