Figure 4
From: Contrasting immune responses mediate Campylobacter jejuni-induced colitis and autoimmunity
Colon cytokine-lymphocyte subset analysis. Interleukin (IL)-10−/− mice were inoculated with Trypticase Soy Broth (TSB) or C. jejuni and killed at indicated day after inoculation. Single-cell suspension was prepared from colon. T cells (a), innate lymphoid cells (ILCs) (c), and natural killer (NK) cells (b) were analyzed for interferon (IFN)-γ and IL-17 or IFN-γ and IL-22 production by intracellular cytokine staining and flow cytometry following brief restimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin in presence of brefeldin A. Double positives were relatively rare and data presented here represent total positive. Dead/dying cells were excluded on the basis of forward and side scatter. Data are represented as mean±s.e.m.; two independent experiments with 5–8 mice per group per time point. Infected group for each time point was compared with control group pooled for each time points by Kruskal–Wallis test and Dunn’s post test. Proportion of IL-17+ or IL-22+ cells from CD3+CD4-cells (which are likely CD8+) was not significantly different at any time point, and is not shown.
