Figure 6
From: Contrasting immune responses mediate Campylobacter jejuni-induced colitis and autoimmunity
Role of Thy-1+ lymphocytes in C. jejuni-mediated colitis. Interleukin (IL)-10−/− mice were orally inoculated with C. jejuni or Trypticase Soy Broth (TSB) and injected with α-Thy-1 or CIgG twice weekly starting 3 days before inoculation, and killed at days 23–24 after inoculation. At the time of necropsy, the ileocecocolic junction (junction of ileum, cecum, and colon) was fixed in formalin and embedded in paraffin. (a) Hematoxylin and eosin (H&E) sections were scored in a blinded manner. (b) Colon leukocytes were prepared and analyzed for indicated populations by flow cytometry. (c) Mice were bled at time of necropsy, and plasma was analyzed for indicated antibody subtypes reactive against C. jejuni antigen. (d) In all, 5 mm of proximal colon was washed and incubated in 0.5 ml tissue culture media for 48 h. Supernatant media was clarified and analyzed for indicated cytokines, as well as other cytokines from Figure 1, which were not significantly different. Bar indicates the median, n=10 mice per group. Kruskal–Wallis test followed by Dunn’s post test. ****P⩽0.0001. OD, optical density.
