Figure 6
From: Activation of C3a receptor is required in cigarette smoke-mediated emphysema
Detection of complement 3 in human and mouse emphysematous lungs. (a) Representative fresh frozen lung tissue obtained from emphysema or control subjects were stained with anti-C3 or non-immune control antibodies (far right panel). Green fluorescence was achieved by Alexa Fluor 488 (AF488)-conjugated secondary antibody and blue fluorescence indicated DAPI nucleus staining. Figures show overlay of green and blue fluorescence. Scale bar: 50 μm. Green arrows indicate C3 deposition. (b) Representative paraffin-embedded lung tissue from mice exposed to 6 months of cigarette smoke or air that were stained with anti-C3 or non-immune control antibodies (far right panel). Green fluorescence was achieved with AF488-conjugated secondary antibody and blue fluorescence resulted from DAPI nucleus staining. Figure shows overlay of green and blue fluorescence. Scale bar: 20 μm. Green arrows indicate C3 deposition. (c) Plasma from control (n=18) and emphysema patients (n=22) were used in an ELISA to measure C3 concentration. **P<0.01 as determined by the Student t test. (d) Correlation of plasma C3 concentration compared with FEV1/FVC%. Solid dots represent individual patients with emphysema and open dots represent individual control patients. P value and r2 were obtained by linear regression model. Results are represented as mean±s.e.m. ANOVA, analysis of variance; BMDCs, bone marrow-derived dendritic cells; C3, complement protein 3; DAPI, 4',6-diamidino-2-phenylindole; FVC, forced vital capacity.
