Figure 4
From: The Axl receptor tyrosine kinase is a discriminator of macrophage function in the inflamed lung
Constitutive binding of Gas6 to the surface of airway macrophages through specific interaction with Axl. (a) Flow cytometric analysis of Axl and Gas6 co-expression on airway macrophages. Flow cytometric analysis of (b) Axl and (c) Gas6 expression on airway macrophages isolated from wild-type (WT) or Axl−/− mice. (d) Relative mRNA expression of Gas6 in purified peritoneal macrophages, lung monocyte-macrophages (mono-mac), and airway macrophages. Symbols represent data from individual mice and lines represent the mean value. (e) Relative mRNA expression of Gas6 in M-CSF- or GM-CSF-differentiated BMDMs. (f and g) Analysis of Axl-dependent binding of Gas6 to M-CSF- or GM-CSF-differentiated BMDMs isolated from WT or Axl−/− mice. BMDMs were left untreated or were incubated with recombinant Gas6 for 1 h and then surface levels of (f) Axl and (g) Gas6 were analyzed by flow cytometry. (f) Specific staining, solid line/open. Isotype control, dotted line/shaded. (g) +Gas6, solid line/open. −Gas6, dotted line/shaded. Data are representative of at least two independent experiments with four mice. **P<0.01 vs. corresponding group; unpaired t-test.
