Figure 6

Axl induction is triggered by immune responses to viruses. (a) Relative mRNA expression of Axl in peritoneal macrophages isolated from naïve mice measured after 24 h stimulation with p(I:C) (10 μg ml⁻¹) or IFN-α (100 ng ml⁻¹). (b) Flow cytometric analysis of Axl expression on peritoneal macrophages stimulated for 48 h with p(I:C) (10 μg ml⁻¹) or IFN-α (100 ng ml⁻¹). Specific staining, solid line/open. Isotype control, dotted line/shaded. (c) Relative mRNA expression of Axl and (d) Western blot analysis of Axl protein expression in M-CSF-differentiated bone marrow-derived macrophages (BMDMs) stimulated with p(I:C) (10 μg ml⁻¹) or IFN-α (100 ng ml⁻¹) for 24 h. (e) Relative mRNA expression of Axl in human monocyte-derived macrophages (MDMs) measured 6, 24, and 48 h after p(I:C) (50 μg ml⁻¹) stimulation. (f) Western blot analysis of Axl protein expression in human MDMs stimulated with p(I:C) (50 μg ml⁻¹) for 48 h. (g) Relative mRNA expression of Axl in human MDMs after 6 and 24 h stimulation with IFN-α (1,000 U ml⁻¹). (h) Relative mRNA expression of Axl in human airway macrophages stimulated with p(I:C) (1 μg ml⁻¹) for 4 h. (i) Western blot analysis of Axl protein expression and STAT1 phosphorylation in human MDMs stimulated with p(I:C) (50 μg ml⁻¹) for 48 h in the absence or presence of an anti-IFNAR-neutralizing antibody (5 μg ml⁻¹). Quantitative PCR data are expressed as the mean relative Axl expression±s.e.m. of three or four individual mice (a,c) or donors (e,g,h). Protein expression data are representative of three or four mice (b,d) or donors (f,i).

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