Figure 7
From: The Axl receptor tyrosine kinase is a discriminator of macrophage function in the inflamed lung
Increased severity of viral lung disease in influenza-infected Axl−/− mice despite efficient clearance of viruses. (a) Change in body mass of wild-type (WT; closed symbol) and Axl−/− (open symbol) mice infected with 7.5 p.f.u. influenza. Amount of interleukin (IL)-6 (b) and chemokine (C-C motif) ligand 2 (CCL-2) (c) in the bronchoairway lavage (BAL) fluid recovered on day 12 post influenza infection from WT and Axl−/− mice. (d) Analysis of viable cells in the BAL from WT and Axl−/− mice recovered on day 12 post influenza infection and counted using trypan blue exclusion. (e–g) Flow cytometric analysis of numbers of (e) neutrophils, (f) CD4+ T cells, and (g) CD8+ T cells in the BAL from WT and Axl−/− mice on day 12 post influenza infection. (h) Influenza genomic mRNA copies recovered from the total lung of WT and Axl−/− mice on days 4 and 8 post influenza infection. (i and j) Flow cytometric analysis of percentage of (i) early (Annexin V+ propidium iodide (PI)−) and (j) late (Annexin V+PI+) apoptotic lymphocytes in the in the BAL from WT and Axl−/− mice on day 10 post influenza infection. (k) Amount of nucleosomes released in the BAL fluid recovered from WT and Axl−/− mice on day 0 (naïve) and day 12 post influenza infection. (l) Efficiency of uptake of apoptotic thymocytes by WT (filled bar) and Axl−/− (open bar) airway macrophages measured by flow cytometry. (a–g and k) are representative of two or three independent experiments with 9–12 mice per group. (i,j) Data from one experiment with 10 mice per group. (h,l) Representative of two independent experiments with four or five mice per group. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 vs. corresponding group; unpaired t-test.
