Figure 3
The maturation phenotype of small intestinal invariant natural killer T (iNKT) cells is distinct from their lymphoid counterparts. (a) CD4 and CD8 expression on iNKT cells were analyzed by flow cytometry. Frequencies are shown as a percentage of iNKT cells. Adult peripheral blood mononuclear cells (PBMCs) were included as a control. ***P≤0.001, **P≤0.01, P*≤0.05; one-way analysis of variance analysis between fetal small intestine, spleen, and mesenteric lymph node (MLN). (b) Markers of lymphocyte maturation were analyzed by flow cytometry on fetal iNKT cells (shaded histograms). The open histograms represent fluorescence minus one (FMO) controls. (c) Frequency of iNKT cells expressing selected cell surface markers represented as box plots. Box and whisker plot show median, interquartile range, and the 25th to 75th percentile. ***P≤0.001, **P≤0.01, P*≤0.05. n=4–8.
