Figure 5
Cytokine production by fetal invariant natural killer T (iNKT) cells. (a) Mononuclear cells in fetal tissues and adult peripheral blood mononuclear cells (PBMCs) were stimulated with α-GalCer for 8 h in the presence of brefeldin A and analyzed by intracellular cytokine staining. (a) The proportion of cytokine-producing fetal iNKT cells producing interferon (IFN)-γ and tumor necrosis factor (TNF)-α are shown in contour plot (≥100 iNKT events were analyzed), representative fluorescence-activated cell sorting (FACS) plots are shown. (b) The percentage of TNF-α+ and (c) IFN-γ+TNFα+ iNKT cells are presented as box plots, n=4–6. Box and whisker plot show median, interquartile range, and the 25th to 75th percentile. **P≤0.01, *P≤0.05.
