Figure 4
Overnight homing of activated/memory α4−/− T cells to the intestinal lamina propria (LP) is reduced. CD4+ T cells from spleens of α4−/− and α4+/+ mice were isolated, labeled with carboxyfluorescein succinimidyl ester (CFSE), and adoptively transferred into recipients. After an overnight homing (∼18 h), cells from the indicated tissues were isolated for flow cytometry analysis and homing index (HI) calculations were calculated as described in the Methods section. (a) Representative contour plots (initially gated on viable CD4+ cells) show percentages of α4−/− and α4+/+ T cells in the indicated tissues. Peripheral LNs (PLNs) were pooled from mandibular, cervical, and axillary LNs. (b, c) Scattergraphs show homing indices from three mice per group that were calculated based on the percentages shown in (a) and normalized to the input ratio of α4−/− and α4+/+ T cells. Line represents mean values. (d) Activation status of T cells isolated from the indicated organs after an overnight homing.
