Figure 6
CD4+CD45RBhigh T cells lacking β1 integrins (β1−/−) produce severe colitis. (a) Blinded histopathological scores of colons from recipient immunodeficient recombination activating gene-1-deficient (RAG-1−/−) mice injected with β1+/+ (black circles) or β1−/− (white circles) T cells. (b) Representative hematoxylin and eosin (H&E)-stained images of distal colons were taken at × 100 magnification. (c) Absolute numbers of CD4+ T cell isolated from spleen, mesenteric lymph nodes (MLNs), colonic lamina propria (LP), and bone marrow (BM) of colitic mice. Pooled data from at least three independent experiments are shown. (d) Representative dot plots showing intracellular cytokine staining for interferon-γ (IFN-γ) and interleukin-17 (IL-17) by colon LP T cells isolated from colitic mice. Graphs shown were initially gated on viable CD4+ T cells. (e) Percentages of cells as shown in (d) pooled from 5 to 7 individually analyzed animals from at least two independent experiments. (f) Expression of integrin molecules on surface of CD4+ T cells isolated from colitic mice and analyzed using flow cytometry. Scatter plots show median fluorescent intensity (MFI) values. Shown are combined data with three animals from a representative experiment that was repeated twice. For all scatter plots, each circle represents an individual mouse; bars represent mean values per group. Significant differences between β1+/+ and β1−/− groups are indicated as*P<0.05 and ***P<0.001.
