Figure 2
From: Foxp3+ regulatory T cells promote lung epithelial proliferation
Epithelial proliferation during acute lung injury (ALI) resolution is impaired in regulatory T (Treg) cell-depleted mice. Treg-depleted mice have decreased levels of epithelial proliferation. (a) Foxp3gfp or Foxp3DTR mice (n=5–9 per group) were challenged with intratracheal lipopolysaccharide (IT LPS) (day 0) and/or diphtheria toxin (DT; 50 or 10 μg kg–1) administered at day −2, −1, 1, 3, and 5 and collected at day 7 post LPS. (b) Representative lung sections were stained with hematoxylin and eosin to reveal morphologic changes 7 days after IT LPS±DT. Original magnification, × 40. (c–f) Total lung cell count (c), total CD326+ cell numbers (d), proliferating epithelial cell (CD326+ki-67+) numbers (e), and percentage of proliferating CD326+ cells compared with total CD326+ cells (f) were determined by flow cytometry. P-values determined by Mann–Whitney test.
