Table 2 Expression analysis of fibrosis mediators in the dextran sodium sulfate model

From: Inhibition of a novel fibrogenic factor Tl1a reverses established colonic fibrosis

 

WT

Pre-Tx Co

Isotype Co

Tl1a Ab—20 mg kg −1

 

% β-Actin

% β-Actin

% β-Actin

% β-Actin

P vs.

 

n =6

n =5

n =5

n =5

WT

Pre-Tx

Isotype

col1a1

0.54±0.45

0.55±0.36

0.67±0.45

0.30±0.17

NS

NS

NS

col1a2

0.67±0.26

1.20±0.94

1.19±0.93

0.63±0.31

NS

NS

NS

col3a1

35.79±10.95

38.64±18.02

35.18±9.74

23.28±3.47

0.044

NS

0.036

col4a1

2.60±1.08

2.62±1.37

2.70±0.54

1.83±0.10

NS

NS

0.010

Tgfβ1

0.21±0.06

0.38±0.15

0.43±0.03

0.22±0.04

NS

0.041

6.943E-05

Ctgf

0.97±0.27

1.1±0.32

1.14±0.36

0.84±0.21

NS

NS

NS

Igf1

0.48±0.18

0.85±0.63

1.09±0.52

0.65±0.28

NS

NS

NS

Pten

0.004±0.003

0.008±0.004

0.012±0.003

0.008±0.002

NS

NS

NS

Il31Ra

3.13±0.65

2.56±0.61

2.67±0.97

2.69±0.31

0.020

NS

0.046

Mmp2

0.52±016

0.60±0.29

1.21±0.38

0.52±0.25

NS

NS

0.007

Mmp3

0.03±0.015

2.07±3.70

1.91±0.10

0.42±0.27

0.003

NS

1.48E-05

Mmp13

0.05±0.011

0.61±1.07

1.02±0.21

0.21±0.15

0.014

NS

0.0003

Timp1

0.04±0.024

0.32±0.37

0.33±0.06

0.15±0.07

0.003

NS

0.004

Timp2

1.02±0.32

0.88±0.17

1.12±0.11

0.74±0.14

NS

NS

0.005

  1. Ab, antibody; ctgf, connective tissue growth factor; Igf1, insulin-like growth factor-1; mmp, metalloprotease; NS, not significant; timp, tissue inhibitors of metalloprotease; tgfβ1, transforming growth factor β1; WT, wild type.
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