Figure 1
From: Retinoic acid primes human dendritic cells to induce gut-homing, IL-10-producing regulatory T cells
RA-conditioned DCs express CD103, RALDH2, and RA. RA-conditioned DCs and MoDC were analyzed before activation by FACS for (a) expression of CCR6, CX3CR1, and CD103 and (b) CD83, CD86, and HLA-DR of RA-DCs and MoDCs after 48 h stimulation with (filled histograms) or without (open histograms) poly IC or LPS. (c) Fold induction of IL-12 and fold reduction of IL-10 (compared with MoDC) after 24 h stimulation with LPS or poly IC of MoDCs or RA-DCs. (d) Fold induction (compared with MoDC) of mRNA expression of ALDH1A1 (n=8) and ALDH1A2 (n=11) in MoDC and RA-DC. (e) Aldehyde dehydrogenase activity of MoDC and RA-DC with or without DEAB as determined by Aldefluor assay (n=7). Results are a representative out of four (a), six (b) or the mean±s.e.m. of five (c) or seven (e) independent experiments. *P<0.05, **P<0.01, ***P<0.001. DCs, dendritic cells; DEAB, diethylaminobenzaldehyde; FACS, fluorescence-activated cell sorting; IL, interleukin; LPS, lipopolysaccharide; MoDC, monocyte-derived DC; poly IC, polyinosinic:polycytidylic acid; RA, retinoic acid.
