Figure 1
From: Dendritic cell CD83 homotypic interactions regulate inflammation and promote mucosal homeostasis
Knockout of CD83 in dendritic cells (DCs) exacerbates colitis. (a) Fluorescence-activated cell sorting (FACS) plots gated on T-cell receptor β+ lymphocytes in the spleen (see Supplementary Figure S2b). Cd83fl/fl;Cd11c-Cre mice had 48.6% CD4-positive T cells; Cd83wt/wt;Cd11c-Cre mice had 48.1% CD4-positive T cells in the spleen. (b, c) DCs were gated on CD11c+ and MHCII+ (see Supplementary Figure S2a). (b) Histograms showing relative expression of CD83 on splenic DCs of Cd83wt/wt;Cd11c-Cre (black line) and Cd83fl/fl;Cd11c-Cre (dashed line) mice. Solid gray histogram shows staining of isotype control on Cd83wt/wt;Cd11c-Cre DCs. (c) FACS plots of DCs isolated from the colon lamina propria of Cd83wt/wt;Cd11c-Cre and Cd83fl/fl;Cd11c-Cre mice. Mice had similar numbers of two main DC subsets, which differentially express CD103 and CD11b. (d) Colitis survival in Cd83fl/fl;Cd11c-Cre (n=7) and Cd83wt/wt;Cd11c-Cre mice (n=6) treated with 3.5% dextran sodium sulfate. Cd83 conditional knockout animals had significantly decreased survival (Log-rank test, *P=0.0186). (e) Percentage of body weight retained following infection. Body weight at day 8 was significantly lower in Cd83fl/fl;Cd11c-Cre mice compared with Cd83wt/wt;Cd11c-Cre littermates. Data presented as mean±s.d. (*P=0.0025). (f) Incidence of frank blood surrounding the anus of animals. In all, 100% of Cd83fl/fl;Cd11c-Cre mice had frank blood surrounding the anus by day 7. Frank blood was not observed in Cd83wt/wt;Cd11c-Cre littermates. (g) Histology scores at day 8 postinfection. Colitis score was significantly increased in Cd83fl/fl;Cd11c-Cre mice (*P=0.0159). Each dot represents a single animal.
